Anti-Mouse CD3 (Clone 17A2) – Recombinant <i>in vivo</i> Functional Grade (C2457-GRP)

CD3 is an invariant antigen of the T cell receptor (TCR) 1. The CD3/TCR complex is composed of a ⍺β or γ? TCR heterodimer noncovalently associated with invariant CD3 dimers ?γ, ??, and ?? in a 1:1:1:1 stoichiometry. The TCR mediates recognition of antigenic peptides bound to major histocompatibility complex (MHC) molecules on antigen-presenting cells, while the CD3 portion of the complex transduces activation signals to the T cell nucleus. Together, TCR and CD3 molecules initiate protective immunity against microbes and cancers. 17A2 has been explored as a treatment against graft-versus-host disease 2,3. 17A2 leads to T cell depletion in vivo that is associated with a significant reduction in B cell population 4. Additionally, 17A2 is less mitogenic that some other anti-CD3 antibodies (i.e., 145-2C11 hamster IgG and KT3 rat IgG2a) 3. FcγRI may be involved in 17A2 activity 5. 17A2 was generated by immunizing female Sprague-Dawley rats with homogenized γ? TCR+ T-T hybridoma cells (D1 line) 6. The resulting rat cells were fused to P3X63-Ag8-653 non-secreting mouse myeloma cells and hybridomas were screened for the secretion of IL-2 in the presence of D1 cells.