Anti-Mouse CD29 [Clone KMI6] - Purified <i>in vivo</i> GOLD™ Functional Grade (C1101-GRP)

Stromal cells are needed for the early steps of B lymphocyte differentiation and proliferation1. CD29, also known as integrin beta 12, is a 110 kDa protein discovered due to its expression on bone marrow stromal cells, at cell surface areas that interact with undifferentiated lymphoid cells1. Integrin beta 1 is a member of a family of alpha beta heterodimers that act as cell surface receptors for extracellular matrix proteins3, including collagen, fibronectin, fibrinogen, laminin, VCAM1, and vitronectin among others4. CD29 is also involved in sperm-egg fusion. Through these interactions, CD29 regulates and/or promotes cell adhesion, laminin matrix deposition, endothelial cell motility, angiogenesis, osteoblast compaction, the formation of mineralized bone nodules, and cytokinesis. KMI6 was raised against the B cell-supportive stromal cell line BMS21. LOU/MN rats were immunized with BMS2 cells and subsequently spleen cells were fused with murine Sp2/0 for hybridoma generation. Antibody recognition is insensitive to the glycosylation state but is lost when the CD29 protein is reduced for Western blotting2. As such, the epitope is likely conformation-dependent. KMI6 was used to identify CD292. KMI6 can enhance cell adhesion of fibronectin to CD4 -8- thymocytes3.