Anti-Mouse CD226 [Clone 10E5] - Purified <i> in vivo </i> GOLD™ Functional Grade (C2481-GRP)

Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signaling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T- lymphocyte and natural killer (NK) cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK- mediated killing of immature dendritic cells 2 and acts as an important costimulatory molecule in Th1 cell activation and effector function3. CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptor NECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding. mCD226 is a marker for mature T cells2 and is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. 10E5 was generated by immunizing Lewis and Lou/M rats with established murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice3. The resulting panel was screened on polarized Th1 and Th2 T cell clones and cell lines to find an antibody that selectively recognizes only Th1 clones. 10E5 specifically recognizes CD226 on the surface of Th1 cells. Additionally, 10E5 inhibits Ag-specific T cell expansion, reducing the expansion of PLP 139-151-specific Th1 cells producing IFN-γ. In vivo treatment with anti- CD226 clone 10E5 inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease.