Anti-Mouse CD1d (CD1.1) - Purified <i>in vivo </i>GOLD™ Functional Grade (C2501-1mg)

CD1d is a non-classical major histocompatibility complex (MHC) molecule that presents glycolipid instead of peptide antigens1 . CD1d is non-MHC-encoded, heavily glycosylated, and a surface protein associated with β2-microglobulin2 . CD1d is present on invariant natural killer (iNKT) cells, where it elicits an immune response upon ligation with the T cell receptor and agonist α-galactosylceramide (α-GalCer )1. iNKT cells activated by α-GalCer secrete cytokines and undergo proliferation. Additionally, α-GalCer can induce anti-tumor immunity. CD1d can also interact with the iNKT agonist β-mannosylceramide (β-ManCer). 20H2 was generated by immunizing male Wistar rats with C57BL/6 thymocytes, boosting three times with a RBL-CD1.1-transfected rat cell line, injecting once with BALB/c thymocytes, and finally injecting with CR1-CD1.1 cells2 . The resulting spleen cells were fused with Sp2/0 cells for hybridoma production. 20H2 cross-reacts with CD1.2-transfected cells and binds to an epitope in the vicinity of the CD1d antigen-binding groove. 20H2 blocks T cell recognition of CD1d2 and can be used to block β-ManCer and α-GalCer agonistic activity1 . Blocking with 20H2 may also attenuate non-allergic asthma3 and ameliorate some aspects of neurodegenerative pathology in mouse4.