Anti-Mouse CD183 (Clone CXCR3-173) - Purified <i> in vivo </i> GOLD™ Functional Grade (C795-GRP)

CXCR3 (CD183) is a chemokine receptor that binds to three ligands, CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (ITAC), that are induced by IFNγ, -α/β, or other proinflammatory cytokines1. CXCR3 is important for natural killer (NK) cell-dependent priming of CD4+ T cells in lymph nodes2, host responses to infection3, and CD4+ T cell responses to allografts4,5. CXCR3-173 was generated by immunizing Armenian hamsters with a peptide sequence unique to mouse CXCR3 which encompasses amino acids 1-376,7. Hamsters were tested by ELISA for seropositivity against CXCR3 peptide and hybridomas were generated, screened by FACS, purified, and tested for: staining, CXCR3 blockade in vitro, and endotoxin levels6. CXCR3-173 detects the native form of CXCR3, and therefore does not work in Western blotting. CXCR3-173 has potential use in immunotherapeutic approaches to inhibit transplant rejection and immune related diseases6. In vitro, CXCR3-173 blocks chemotaxis in response to CXCL10 or CXCL11 but not CXCL9. In vivo, CXCR3-173 prolongs both cardiac and islet allograft survival in a manner further enhanced by rapamycin.