Anti-Ly49C (Clone 4LO3311) - Purified <i> in vivo </i> GOLD™ Functional Grade (L244-GRP)

Ly49 receptors are homodimeric type II C-type lectin-like membrane glycoproteins encoded by a family of highly polymorphic genes in the mouse natural killer (NK) gene complex1. Ly49 receptors recognize class I major histocompatibility complex-I (MHC-1) and MHC-1 like proteins. Ly49C is an inhibitory member of the Ly-49 superfamily. Inhibitory Ly49 receptors are involved in NK cell education1 and play a role in controlling viral infection2. Additionally, Ly49C decreases the activation threshold of NK cells by inhibiting H2-Kb 3. Ly49C is expressed on NK, uterine NK, NKT, and CD8+ Tregs cells1. Ly49C is closely related to Ly49F, Ly49I, and Ly49H3. 4LO3311 was generated by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells4. Hybridoma lines were prepared by fusing immune spleen cells with non-secreting P3X63-Ag8.653 myeloma cells and subsequently screened for production of anti-NK antibodies. Isotype was determined by double-immunodiffusion analysis of hybridoma supernatants using rabbit monospecific anti-mouse immunoglobulins. 4LO3311 is of IgG3, κ isotype. 4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain5. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.