Anti-Human Lamin A/C (Clone 4C11) - Biotin (L352-GRP)

The nucleoplasm is separated from the cytoplasm by a bilayer nuclear envelope that consists of outer and inner nuclear membranes, nuclear pore complexes, and the nuclear lamina, which is a two-dimensional matrix composed mainly of type V intermediate filament proteins called lamins 1. Lamins are encoded by a family of three genes that collectively produce lamins B1, B2, B3, AΔ10, C2, and A/C. Lamin A/C plays a variety of roles in the nucleus including, but not limited to: nuclear mechanical stability, structural maintenance, positioning, chromatin organization, epigenetic regulation, transcription, DNA replication, cell cycle progression, differentiation, migration, and polarization. Additionally, lamin A/C is variably expressed in human immune cells during different stages of cell development and activation states. 4C11 was raised against the wild-type Ig-fold domain of lamin A/C (aa 430–545)2. This domain is located in the C-terminal globular domain of the protein’s tripartite structure. A BALB/c mouse was immunized with antigen and splenocytes fused with myeloma cell line X63Ag8.653. 4C11 detects both lamins A and C.