Anti-Human IgG3 [Clone HP6050] - Purified No Carrier Protein (I-1231-250ug)

Humans produce five classes of immunoglobulins, IgM, IgD, IgA, IgE, and IgG1. IgG is the most abundant class1 and functions in the humoral immune response2. There are four subclasses of IgG, and they are numbered according to decreasing abundance as IgG1 through IgG4. The four subclasses are highly conserved and consist of four polypeptide chains: two identical heavy chains and two identical light chains1. While the general structure is very similar between IgG subclasses, differences in their constant regions in the hinge and upper CH2 domain as well as in glycosylation affect their binding to antigens (e.g. pathogenic proteins, polysaccharides, allergens), C1q and Fc receptors, leading to differences in functionality. As a result, each IgG subclass has a unique response skewed to specific antigens. IgG3 is a pro-inflammatory antibody that potently induces effector function1. However, IgG3 has a relatively shorter half-life compared to other IgG antibodies, and this is thought to deter an excessive inflammatory response. IgG3 is also unique relative to the other IgG subclasses in that its hinge is elongated due to duplications of the hinge exon. Additionally, many IgG3 allelic forms exist. IgG3 antibodies are the first subclass to respond to viral infection, with IgG1 following. HP6050 was generated by immunizing a BALB/c mouse with purified human myeloma protein3. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.