Anti-Human CXCR2 (Clone 4D1.5.7) - Purified No Carrier Protein (C3117-500ug)

Chemokines (chemoattractant cytokines) play an important role in immune and cancer cell migration 1, metastasis, leukocyte trafficking to inflammatory sites 2, angiogenesis, neutrophil chemotaxis and activation 3 as well as cutaneous burn wound repair. At least 20 chemokine receptors have been identified, among them CXCR2 (IL-8RB). CXCR2 is an interleukin (IL)-8 (CXCL8) receptor that also binds to the CXC chemokines CXCL1 (GROα), CXCL2, CXCL3, CXCL4, CXCL5 (ENA78), CXCL6, and CXCL7 (NAP-2) 1. CXCR2 is also activated by MIF (macrophage migration inhibitory factor). Ligand binding occurs via the extracellular N- terminus. CXCR2 can exist as a homodimer or heterodimer with CXCR1 1. Many cancers that have poorer patient prognosis are associated with increased CXCR2 expression. 4D1.5.7 (also known as HB-11495) was generated by immunizing mice with 293 cells that express CXCR2 4. Hybridomas were selected based on their ability to bind human neutrophils. In flow cytometry assays, 4D1.5.7 bound to cells expressing CXCR2 but not to cells expressing CXCR1. ELISA experiments were subsequently used to identify residues 1-18 of the N-terminal as containing the binding site. Subsequent epitope mapping concluded that 4D1.5.7 recognizes residues 6-10 (FEDFW) of CXCR2 as its binding epitope 2. 4D1.5.7 can inhibit IL-8 binding to cells expressing CXCR2 by up to 90% and to human neutrophils by up to 50% 4.