Anti-Human CD3 x CD19 (Blinatumomab) – Fc Muted™ (C2535-500ug)

Blinatumomab is a Bispecific T cell Engager (BiTE) antibody developed as a cancer immunotherapeutic drug1,2,3,4. Blinatumomab induces apoptosis of target B cells by binding simultaneously to the C19 surface antigen of all B cells (healthy and malignant) as well as the epsilon subunit of the CD3 invariant antigen of the T cell TCR (T cell receptor)4. Binding is achieved via two large single-chain variable fragments arranged in tandem, with the CD19-binding fragment at the N-terminal and the CD3 binding fragment at the C-terminal. The fragments are linked by a flexible, non-immunogenic, non-glycosylated five amino acid peptide (four glycine and one serine), which confers a high degree of rotational flexibility to facilitate simultaneous epitope binding. In this way, blinatumomab targets malignant B cells for apoptosis via CD19, a B-lymphocyte-specific receptor responsible for promoting activation and differentiation of normal B cells that functions as a costimulatory molecule of the B cell receptor2. Blinatumomab binding forces the colocalization of cytotoxic T lymphocytes and B cells expressing CD194. A structurally normal cytolytic immune synapse is formed, and, in T cells, activation events trigger the delivery of granzyme and perforin into the synaptic space, inducing apoptosis of the targeted B cells. Recruitment and activation of T cells occurs after the second arm of blinatumomab binds to the target cell antigen. An activated T cell can kill several B cells. Blinatumomab is a B lineage-specific antitumor mouse monoclonal antibody4. The CD19-targeting fragment is derived from the parental murine monoclonal antibody HD37, while the CD3-binding fragment is derived from the parental murine monoclonal antibody L2K-071,3,4. Blinatumomab is only one-third the size of traditional antibodies at 504 amino acids and a molecular weight of 55 kDa4. Other names for blinatumomab are MT103, MEDI‐538, bscCD19xCD3, and AMG103. Blinatumomab is a non-glycosylated fusion protein.