Anti-Human CD28 (Clone 9.3) - Biotin (C884-GRP)

CD28, a glycoprotein weighing 44 kDa is a member of the Ig superfamily and plays a vital role, in the activation and survival of T cells1, 2 by enhancing the production of interleukin and the activity of T cells. CD28 is unique among B7 receptors as it is consistently expressed on T cells. The interaction between CD28 and B7 is crucial for preventing T cells from becoming anergic and unable to respond to antigen/MHC complexes highlighting its role in an immune response3-6. The clone 9.3 has gained recognition for its ability to stimulate the growth of human T cells in vitro making it a valuable tool for research focused on studying T cell activation and immune responses. It was developed by immunizing mice with human T cells. It has demonstrated effectiveness in inducing T cell activation and proliferation when combined with TPA thereby influencing IL 2 receptor expression and IL 2 production7. This clone can be utilized for staining a portion of T cells through immunofluorescence methods and has proven successful in immunoprecipitating CD28 from Jurkat cells8. Given its ability to activate T cells and its reactivity with CD28, the 9.3 clone stands as a resource for research.