Anti-Human CD16 (Clone 3G8) - Purified <i>in vivo </i>GOLDâ„¢ Functional Grade
Anti-Human CD16 (Clone 3G8) - Purified <i>in vivo </i>GOLDâ„¢ Functional Grade (C2858-GRP)
Products
Description
CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.
Specifications
| Manufacturer Cat# | C2858 |
|---|---|
| Concentration | ≥ 5.0 mg/ml |
| Formulation | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Isotype | IgG1 κ |
| Clone | 3G8 |
| Antibody Type | Monoclonal |
| Applications | B, CyTOF®, FC, IHC FF, IP |
| Antigen | CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts. |
| Storage | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Protein | CD16 |