Alpha-actinin protein - rabbit skeletal muscle (AT01-GRP)

Product Uses Include

• Discover and characterize α-actinin binding proteins
• Positive control of actin binding assays
• Positive control for actin bundling assays
  
  

Material
α-actinin has been purified from rabbit skeletal muscle. The protein is supplied as a lyophilized powder and upon reconstitution, will be in the following buffer: 4 mM Tris-HCl pH 7.6, 4 mM NaCl, 20 μM EDTA, 1% (w/v) sucrose, and 0.2% (w/v) dextran. The lyophilized product is stable at -70°C for at least 2 years. AT01 should be resuspended in nanopure water containing 1 mM β-mercaptoethanol or DTT to the desired working concentration. 

Purity
Purity is determined by scanning densitometry of the protein on an SDS-PAGE gel. α-actinin is >90% pure. 

Biological Activity
At 2.5 µM (0.25 mg/ml) α-actinin and 25 µM (1.0 mg/ml) F-actin at pH 7.0 and 24°C, approximately 70% of the α-actinin will co-sediment with F-actin after centrifugation at 150,000 x g for 1 h.