AF 555 alkyne (LP1K8B0)

AF 555 alkyne is a yellow fluorescent dye containing a terminal alkyne group for bioorthogonal conjugation via copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The dye reacts selectively with azide-modified biomolecules, forming stable triazole linkages under mild aqueous conditions. AF 555 alkyne absorbs at ~552 nm and emits bright fluorescence at ~566 nm. The dye is characterized by high brightness, good photostability, and high water solubility, making it suitable for fluorescence microscopy, flow cytometry, and high-content screening. Its excitation profile is compatible with standard 532–561 nm laser lines. The reagent is commonly used for post-labeling of azide-functionalized proteins, glycans, nucleic acids, lipids, and other biomolecules introduced by metabolic labeling or chemical modification.