Actin protein (chicken gizzard muscle, >99% pure)

Product Uses Include

• Identification and characterization of smooth muscle actin binding proteins
• In vitro smooth muscle actin polymerization studies
  
  

Material:
Smooth muscle actin has been purified from chicken gizzard.  The isotype composition of chicken gizzard smooth muscle actin is approximately 80% γ smooth muscle and 20% β cytoplasmic actin (1).  Chicken gizzard smooth muscle actin has an approximate molecular weight of 43 kDa and is provided as a lyophilized white powder. 

Purity:
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel.  Smooth muscle actin is >99% pure. 

Biological Activity:
The biological activity of smooth muscle actin is determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay.  Stringent quality control ensures that >85% of the smooth muscle actin polymerizes in this assay.