Acetyl-Lysine Affinity Beads - AAC03 (AAC03-beads-L)

Cytoskeleton offers two types of Acetyl-Lysine Affinity Beads, Cat # AAC02-Beads and Cat # AAC03-Beads. Both reagents are comprised of mouse anti-acetyl-lysine antibodies that are covalently linked to protein G beads and both enrich a broad range of acetylated proteins. AAC02-Beads and AAC03-Beads can be combined to give a more extensive acetylated protein enrichment profile (Cat # AAC04-Beads is a 1:1 combination of AAC02-Beads:AAC03-Beads). AAC03-Beads has an overlapping but unique specificity profile when compared to AAC02-Beads and may outperform AAC02-Beads when examining a specific target protein, see Detailed methods and Validated Applications for examples. As acetylation generally represents a very small percent of total target protein, utilizing optimized reagents is extremely critical to success. When examining the acetylation state of a new protein of interest (POI), it is recommended to try AAC03-Beads and AAC02-Beads separately to determine which reagent is optimal for your POI.