5-Bromo-4-chloro-3-indolyl-phosphate-p-toluidine-salt research grade (15247.03)

Used in conjunction with NBT (cat. no. 30550) for detection of alkaline phosphatase.Stock solution: 50 mg/ml in 100 % DMF. Store at 4 °C or -20 °C.Staining solution for western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5).