SiR-Tetrazine

SiR-tetrazine is a clickable derivative of SiR. This probe reacts with dienophiles such as trans-cyclooctene (TCO) or bicyclononyne (BCN) derivatives, enabling the preparation of custom SiR probes and conjugates. SiR-tetrazine is particularly useful in combination with unnatural amino acid (UAA) incorporation to prepare site selective protein conjugates. SiR-tetrazine is cell permeable and fluorogenic, it can be used to stain live cells as well as fixed ones. SiR is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with a standard Cy5 filterset.

Properties

λ_abs 652 nm
ε_max 1.0·10⁵ mol^-1·cm^-1
λ_fl 674 nm
MW =641.8 g/mol
CF₂₆₀ = 0.116
CF₂₈₀ = 0.147