SERVALight Vega CL HRP WB Substrate Kit

Highly sensitive enhanced chemiluminescence kit for the detection of immobilized proteins (Western Blot) or immobilized nucleic acids (Southern and Northern Blot) labelled directly with horseradish peroxidase (HRP) or indirectly with HRP-labelled antibodies/streptavidin. The substrate is readily prepared by mixing component A (luminol/enhancer solution) with component B (stabilized peroxide solution) in a one-to-one ratio.• High sensitivity, low picogram limit of detection• Directly compatible with protocols of standard ECL WB substrates of other vendors• Low background, high signal/noise ratio• Working solution is stable for minimum 5 days – reproducible results, less waste• Primary antibody dilution 1:100 -1:5000• Secondary antibody dilution 1:1000 – 1:15.000• Detection can be done by film or CCD imaging equipment