Product Uses Include
- RhoC biochemistry
- RhoC GTPase assays
- RhoC nucleotide exchange assays
- RhoC binding studies
Material
The human RhoC protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is approximately 30 kDa, consisting of the RhoC protein plus a histidine tag in the amino-terminus.
Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-RhoC samples are ~90% pure.
Biological Activity
The biological activity of His-RhoC is determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to sequester magensium ions from the His-RhoC protein, thereby stimulating nucleotide exchange activity. The RhoGEF exchange assay biochem kit (Cat. # BK100) is used to monitor the exchange ability of His-RhoC.
