Recombinant Protein A Sepharose FF Resin

Protein A Sepharose® FF Resin designed for simple, one-step and rapid antibody purification from serum, ascites and tissue culture supernatant such as those derived from static cultures and bioreactors. Recombinant protein A has been coupled to Sepharose® to obtain a stable matrix with a high binding capacity for immunoglobulins via the heavy chain of the FC region (up to 30 mg/ml Human IgG). Antibody samples purified using this affinity resin may be used in a wide range of laboratory procedures such as 1D or 2D polyacrylamide gel electrophoresis, Western Blotting, ELISA etc. Binding affinity varies depending upon the source species and subclass. Specifications Specificity: Protein A affinity antibodies Matrix: Sepharose® Coupled ligand: 3.5 mg Protein A/ml resin Binding capacity (human IgG): 30 mg/ml Bead size: 60 - 165 μm Flow rate: 0.25 - 1 ml/min (recommended) Maximum pressure: 120 - 140 psi Buffer compatibility: Common aqueous buffers from pH 2.5 - 10 Binding buffer example: 1.5 M Glycine/NaOH, 3 M NaCl pH 9.0 Elution buffer example (1): 0.1 M sodium citrate pH 5.5 Elution buffer example (2): 0.2 M Glycine/HCl pH 2.5 Neutralization buffer example: 1 M Tris/HCl pH 9.0 Shipping/delivery: 50 % (v/v) resin suspension in 0.01 % thimerosal Storage: 0.01 % thimerosal at 2 - 8 °C for up to 2 years from manufacture