RalA G-LISA Activation Assay KitRalA G-LISA Activation Assay Kit
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RalA G-LISA Activation Assay Kit

The proprietary RalA G-LISA™ Activation Assaythat is faster, easier and more precise than traditional pull-down methods to measure RalA Activation.

  • Specific for RalA
  • Colorimetric Based
  • Fast Results
  • Linear from 0.5 to 5 ng
  • Flexible format

General Information

The RalA G-LISA™ kit contains a Ral-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Ral is captured by this protein while inactive GDP-bound Ral is removed following washes. The active RalA bound to the wells is detected with a RalA specific antibody. The degree of RalA activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.

Kit Contents - Enough reagents for 96 assays.

  • 96 individual Ral-GTP binding wells
  • Anti-RalA monoclonal antibody
  • Secondary Antibody - HRP
  • RalA control protein
  • Ral Binding Buffer
  • Cell Lysis Buffer
  • Wash buffer
  • Antigen Presenting Buffer
  • Antibody Dilution Buffer
  • HRP Detection and Stop Reagents
  • Precision Red™Advanced Protein Assay (Cat. # ADV02)
  • Protease Inhibitor Cocktail (Cat. # PIC02)
Cat# Size Price Qty Buy
BK129 96 assays £678.60

Additional Information

Property Value or Rating
Product Size 96 assays
Manufacturer Cytoskeleton, Inc
Storage 4°C
References

Papini D. et al. 2015. TD-60 links RalA GTPase function to the CPC in mitosis. Nat. Commun. 6, 7678.

Ribback et al., 2013. Molecular and metabolic changes in human liver clear cell foci resemble the alterations occurring in rat hepatocarcinogenesis. J. Hepatology. http://dx.doi.org/10.1016/j.jhep.2013.01.013.

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