Rac1 G-LISA Activation Assay Kit

The proprietary Rac1 G-LISA™ Activation Assay that is faster, easier and more precise than traditional pull-down methods to measure Rac1 Activation.

• Specific for Rac1
• Colorimetric Based
• Fast Results
• Extremely Sensitive 

General Information

This Rac1 G-LISA™ is a colorimetric based assay and shares the many advantages that all G-LISA™ activation assay have. This assay is specific for Rac1.

The Rac1 G-LISA™ kit contains a Rac-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Rac1 is captured by this protein while inactive GDP-bound Rac1 is removed following washes. The active Rac1 bound to the wells is detected with a Rac1 specific antibody. The degree of Rac1 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates. 

Kit Contents - Enough reagents for 96 assays (Stripwell)

• 96 individual Rac1-GTP binding wells
• Anti-Rac1 antibody (Cat. # ARC03)
• Secondary Antibody - HRP
• Rac1 control protein
• Cell Lysis Buffer
• Wash buffer
• Antigen Presenting Buffer
• Antibody Dilution Buffer
• HRP Detection and Stop Reagents
• Precision Red™ Advanced Protein Assay (Cat. # ADV02)
• Protease Inhibitor Cocktail (Cat. # PIC02)