Product Uses Include
- Rac1 signaling pathway studies
- Rac1 activation assays with primary cells
- Studies of Rac1 activators and inactivators
- Rac1 activation assays with limited material
- High throughput screens for Rac1 activation/inhibition
Introduction
The G-LISA series of Small G-Protein Activation Assays are ELISA based assays with which you can measure the GTP form of small G-proteins from lysates of cells or tissues and all in less than 3 h. The level of activation is measured with luminescence. The Rac1 G-LISA™ Activation Assay (Cat.# BK126) measures the level of GTP-loaded Rac1 protein in cell lysates, this is in contrast to Cat.# BK125 which does not distinguish between activated Rac1,2 or 3. For a kit to measure RhoA activation please check Cat.# BK124.
The Rac1 G-LISA Activation Assay is very sensitive and has excellent accuracy for duplicate samples.
Kit contents
The kit contains sufficient reagents to perform 96 Rac activation assays. Since the Rac-GTP affinity wells are supplied as strips and the strips can be broken into smaller pieces, each kit can be used for anywhere from one to multiple assays. The following components are included in the kit:
- 96 Rac-GTP affinity wells (divisible into 96 individual wells)
- Lysis buffer
- Binding buffer
- Antigen presenting buffer
- Wash buffer
- Antibody dilution buffer
- Anti-Rac1 antibody
- HRP-labeled secondary antibody
- Positive control Rac1 protein
- Protease inhibitor cocktail (Cat. # PIC02)
- Luminescence detection reagents
- Precision Red™ Advanced protein assay reagent (Cat. # ADV02)
- Manual with detailed protocols and extensive troubleshooting guide
Equipment needed
- 96-well plate luminometer
- Multichannel or multidispensing pipettor
- Orbital microplate shaker capable of at least 200 rpm shaking (400 rpm is optimal)
