Source
Profilin antibody is provided as an affinity purified rabbit polyclonal antibody. The antibody has been raised against purified human profilin and it is predicted to have broad species cross reactivity. The positive control protein consists of an extract of bovine thymus that identifies a characteristic profilin band at 14 kDa. The control protein (bovine thymus extract, Cat. # EXT03) is supplied as a lyophilized powder; this should be reconstituted in 500 μl of cold Milli-Q water to 2 mg/ml. After addition of water the protein should be left at room temperature for 5 minutes with occasional pipetting up and down to make sure that the protein has completely dissolved into solution, it can then be stored at -20°C. We recommend running 10 μg of positive control protein in Western blots.
Materials
The profilin antibody (50 μg) is supplied as a lyophilized powder. It is recommended to resuspend the powder in 200 μl of 30% glycerol/water. This gives a concentration of 250 μg/ml in PBS plus 30% glycerol with 0.01% chlorhexidine for preservative.
