Profilin 1 (recombinant human no tag)Profilin 1 (recombinant human no tag)
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Profilin 1 (recombinant human no tag)

Product Uses Include

  • Actin binding studies
  • Actin polymerization studies

Material

Profilin protein has been expressed in E.coli and purified via a 6xHis tag that is later cleaved by protease digestion. Thus the protein does not contian a 6xHis or other tag. The protein is supplied as a lyophilized powder. When resuspended in nanopure water, the profilin will be in the following buffer:  10 mM Tris pH 8.0, 1 mM EDTA, 1 mM DTT, 5% (w/v) sucrose and 1% (w/v) dextran.

The lyophilized protein is stable at 4°C desiccated (<10% humidity) for up to 1 year.

Purity

Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Purity is >95% (see figure 1).

Biological activity
Profilin is tested for biological activity by a binding assay with actin. Profilin is bound to poly-L-proline sepharose beads and incubated with G-actin. The profilin beads bind >90% of the available actin by this procedure.

Cat# Size Price Qty Buy
PR02-A 1 x 100 ug
£156.78
PR02-B 1 x 500 ug
£335.40
PR02-XL2 1 x 1 mg
£715.26

Additional Information

Property Value or Rating
Manufacturer Cytoskeleton, Inc
Storage 4°C

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