H-Ras-His Protein wild type

Product Uses Include

• Ras biochemistry
• Ras GTPase assays
• Ras nucleotide exchange assays
• Ras binding studies

Material
The human H-Ras protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.

The recombinant protein is approximately 28 kDa, consisting of the H-Ras protein plus a histidine tag in the amino-terminus.

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-H-Ras samples are >80% pure.