EnzyChrom™ NADP/NADPH Assay KitEnzyChrom™ NADP/NADPH Assay Kit
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EnzyChrom™ NADP/NADPH Assay Kit

For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.

• Sensitive and accurate. Detection limit 0.1 μM, linearity up to 10 μM NADP+/NADPH in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required. 

• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems’ EnzyChrom™ NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.

Cat# Size Price Qty Buy
ECNP-100 100 Tests £352.15

Additional Information

Property Value or Rating
Product Size 100 Tests
Manufacturer BioAssay Systems
Applications For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism.
Method OD565nm
Samples Cell, tissue extracts etc
Species All
Detection Limit 0.1 μM
Storage -20 °C
Shelf Life 6 months

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