Protein Standards for IEF
To determine the isoelectric points of unknown proteins the pH of focused bands may be measured on the gel using a surface electrode. Quite common is the pI determination via coelectrophoresis of known protein marker mixtures. By simply comparing the position of unknown protein bands to the position of known marker proteins the pI values can be interpolated quite accurately.
SERVA provides ready-to-use protein markers for Isoelectric Focusing, where one standard is applicable to all IEF gels.