Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.
SERVA offer a comprehensive range of products for IEF, featuring Precast Horizontal and Vertical Gels, SERVALYT™ Carrier Ampholytes and Protein Standards for IEF. Accessories for IEF and equipment for Flatbed IEF.