Depending on the type of blotting (tank or semi-dry blotting) and the specific characteristics of the proteins, different buffers may be used for Western Blotting. For small proteins or for tank blotting after SDS PAGE Towbin buffer is recommended.
A fast and easy to use alternative for optimal semi-dry blotting is SERVA’s Xpress Blotting Kit. The buffer system enables in combination with the newly developed alternative for blotting paper, the Blotting Fleece, an efficient simultaneous transfer of high and low molecular weight proteins in only 15 minutes. SERVA's ready-to-use transfer buffers save you time and labor and guarantee best results.