Detection Reagents

Before membrane bound antigens can be detected, unspecific binding sites for antibodies on the transfer membrane have to be blocked. For many applications skim milk powder is an economic alternative to bovine serum albumin (BSA). For Western Blots where skim milk cannot be used, SERVA offers highly purified BSA like Albumin bovine Fraction V, pH 7.0. SERVALight HRP Chemiluminescence Kits are a new family of highly sensitive ready-to-use kits for chemiluminescence detection of membrane bound antigens (Western Blot) or nucleic acid sequences (Southern and Northern Blot), labelled directly with Horseradish Peroxidase (HRP) or indirectly with HRP-conjugated antibodies/streptavidin.
20 Product(s)
  1. 5,5'-Tetramethylbenzidine (35926)

    Starting at: £64.42

    research grade
  2. Albumin bovine Fraction V, pH 7.0 (11930)

    Starting at: £24.29

    standard grade, lyophil.
  3. BCIP/NBT ready-to-use substrate (15246.01)

    £64.42
    Product Size: 100 ML
    for immunohistochemistry and blotting
  4. Chemiluminescence reagent (42582)

    Starting at: £54.91

    for Horseradish Peroxidase
  5. Denhardt's solution, 50x concentrate (39603.01)

    £79.07
    Product Size: 10 ML
    molecular biology grade
  6. Ficoll(R) 400 (39763.02)

    £146.78
    Product Size: 50 G
    molecular biology grade
  7. Luminol (28085.02)

    £33.79
    Product Size: 5 G
    research grade
  8. Nitro Blue tetrazolium chloride (NBT) (30550)

    Starting at: £26.40

    analytical grade
  9. SERVAColor BCIP/NBT Blot Solution (15245.01)

    £160.51
    Product Size: 250 ML
  10. SERVALight Eos (42585)

    Starting at: £76.03

    CL HRP WB Substrate Kit
  11. SERVALight EosUltra (42586)

    Starting at: £100.32

    CL HRP WB Substrate Kit
  12. SERVALight Helios (42587)

    Starting at: £103.49

    CL HRP WB Substrate Kit
  13. SERVALight Polaris (42584)

    Starting at: £73.92

    CL HRP WB Substrate Kit
  14. SERVALight Vega (42588)

    Starting at: £36.96

    CL HRP WB Substrate Kit
  15. Skim milk powder (42590)

    Starting at: £19.01

    for blotting
  16. TMB ready-to-use substrate for Blotting (37070.01)

    £65.47
    Product Size: 100 ML
  17. Tween(R) 20 (37470)

    Starting at: £23.23

    research grade, Ph. Eur., USP