Detection Reagents

Before membrane bound antigens can be detected, unspecific binding sites for antibodies on the transfer membrane have to be blocked. For many applications skim milk powder is an economic alternative to bovine serum albumin (BSA). For Western Blots where skim milk cannot be used, SERVA offers highly purified BSA like Albumin bovine Fraction V, pH 7.0. SERVALight HRP Chemiluminescence Kits are a new family of highly sensitive ready-to-use kits for chemiluminescence detection of membrane bound antigens (Western Blot) or nucleic acid sequences (Southern and Northern Blot), labelled directly with Horseradish Peroxidase (HRP) or indirectly with HRP-conjugated antibodies/streptavidin.
27 Product(s)
  1. 5,5'-Tetramethylbenzidine (35926)

    Starting at: £65.47

    research grade
  2. Albumin bovine Fraction V, pH 7.0 (11930)

    Starting at: £25.34

    standard grade, lyophil.
  3. AquaSpark(TM) Alkaline Phosphatase Substrate (42593.01)

    £107.71
    Product Size: 100µL
    2 mM in DMSO
  4. AquaSpark(TM) Broad Range Phosphatase Substrate (42594.01)

    £109.82
    Product Size: 100µL
    2 mM in DMSO
  5. BCIP/NBT ready-to-use substrate (15246.01)

    £66.53
    Product Size: 100ML
    for immunohistochemistry and blotting
  6. BlueBlock PF (10x) (42591)

    Starting at: £43.30

    for blotting
  7. Bromo-4-chloro-3-indolyl-phosphate-p-toluidine-salt (15247.03)

    £89.76
    Product Size: 500MG
    research grade
  8. Chemiluminescence reagent (42582)

    Starting at: £54.91

    for Horseradish Peroxidase
  9. Denhardt's solution, 50x concentrate (39603.01)

    £80.13
    Product Size: 10ML
    molecular biology grade
  10. Ficoll(R) 400 (39763.02)

    £149.95
    Product Size: 50G
    molecular biology grade
  11. Luminol (28085.02)

    £34.85
    Product Size: 5G
    research grade
  12. Nitro Blue tetrazolium chloride (NBT) (30550)

    Starting at: £26.40

    analytical grade
  13. PBST Buffer (10x) (42597.01)

    £66.53
    Product Size: 1L
    sterile
  14. SERVA VisiBlot Standard I (39260.01)

    £241.82
    Product Size: 500µL
  15. SERVAColor BCIP/NBT Blot Solution (15245.01)

    £163.68
    Product Size: 250ML
  16. SERVALight EosUltra (42586)

    Starting at: £100.32

    CL HRP WB Substrate Kit
  17. SERVALight Helios (42587)

    Starting at: £103.49

    CL HRP WB Substrate Kit
  18. SERVALight Polaris (42584)

    Starting at: £76.03

    CL HRP WB Substrate Kit
  19. SERVALight Vega (42588)

    Starting at: £43.30

    CL HRP WB Substrate Kit
  20. Skim milk powder (42590)

    Starting at: £20.06

    for blotting
  21. TBST Buffer (10x) (42598.01)

    £71.81
    Product Size: 1L
    sterile
  22. TMB ready-to-use substrate for Blotting (37070.01)

    £65.47
    Product Size: 100ML
  23. Tween(R) 20 (37470)

    Starting at: £24.29

    research grade, Ph. Eur., USP