Nucleic acids are usually separated on agarose gels. Because of the consistency of the gel matrix, blotting can be performed as a capillary blot using SSC or SSPE as blotting buffer. The ideal transfer membrane is a nylon membrane with positive surface like Nylon-Bind B.
Proteins are most often separated by acrylamide gel electrophoresis. As these gels are too restrictive for capillary blotting, Western Blots are done by electro transfer.