Blotting

Nucleic acids are usually separated on agarose gels. Because of the consistency of the gel matrix, blotting can be performed as a capillary blot using SSC or SSPE as blotting buffer. The ideal transfer membrane is a nylon membrane with positive surface like Nylon-Bind B. Proteins are most often separated by acrylamide gel electrophoresis. As these gels are too restrictive for capillary blotting, Western Blots are done by electro transfer.
Showing 1-50 of 64 Products
  1. 5,5'-Tetramethylbenzidine (35926)

    Starting at: £65.47

    research grade
  2. Albumin bovine Fraction V, pH 7.0 (11930)

    Starting at: £25.34

    standard grade, lyophil.
  3. Aminocaproic acid (12548.03)

    £33.79
    Product Size: 100G
    analytical grade
  4. AquaSpark(TM) Alkaline Phosphatase Substrate (42593.01)

    £107.71
    Product Size: 100µL
    2 mM in DMSO
  5. AquaSpark(TM) Broad Range Phosphatase Substrate (42594.01)

    £109.82
    Product Size: 100µL
    2 mM in DMSO
  6. BCIP/NBT ready-to-use substrate (15246.01)

    £66.53
    Product Size: 100ML
    for immunohistochemistry and blotting
  7. BlueBlock PF (10x) (42591)

    Starting at: £43.30

    for blotting
  8. BlueBlot Semi-Dry Blotter SD11 (550205.01)

    £2,336.40
    Product Size: 1PIECE
    Blotting Area 11 cm x 11 cm
  9. BlueBlot Semi-Dry Blotter SD17 (550206.01)

    £2,699.40
    Product Size: 1PIECE
    Blotting Area 17 cm x 17 cm
  10. BlueBlot Semi-Dry Blotter SD26 (550207.01)

    £3,821.40
    Product Size: 1PIECE
    Blotting Area 24 cm x 26 cm
  11. Bromo-4-chloro-3-indolyl-phosphate-p-toluidine-salt (15247.03)

    £89.76
    Product Size: 500MG
    research grade
  12. Chemiluminescence reagent (42582)

    Starting at: £54.91

    for Horseradish Peroxidase
  13. Citric acid-Na3-salt-2H2O (38642.02)

    £31.68
    Product Size: 1KG
    analytical grade, Ph. Eur.
  14. Denhardt's solution, 50x concentrate (39603.01)

    £80.13
    Product Size: 10ML
    molecular biology grade
  15. Electrode Set for BB-SD11 (550208.01)

    £1,287.00
    Product Size: 1PIECE
    Blotting Area 11 cm x 11 cm
  16. Electrode Set for BB-SD17 (550209.01)

    £1,841.40
    Product Size: 1PIECE
    Blotting Area 17 cm x 17 cm
  17. Ethanol denaturated 96 % (11096)

    Starting at: £76.40

  18. Ethylenediamine tetraacetic acid Na2-salt (39760.01)

    £30.62
    Product Size: 250G
    molecular biology grade
  19. Fluorobind Membrane, surface PVDF (42571.01)

    £402.34
    Product Size: 1ROLL
    Pore size 0.2 µm, format: 25 cm x 3 m
  20. Glycine (23391)

    Starting at: £31.68

    electrophoresis grade
  21. Glycine (23390)

    Starting at: £20.06

    analytical grade, Ph. Eur., USP
  22. Gravity Blotter (550340.01)

    £1,155.00
    Product Size: 1PIECE
    Blotter Area 14 x 29 cm
  23. Immobilon(TM)-P-membrane (42574.01)

    £807.84
    Product Size: 1 ROLL
    Pore size 0.2 µm, format: 26.5 cm x 3.75 m
  24. Immobilon(TM)-P-membrane (42581.01)

    £717.02
    Product Size: 1ROLL
    Pore size 0.45 µm, format: 26.5 cm x 3.75 m
  25. Luminol (28085.02)

    £34.85
    Product Size: 5G
    research grade
  26. Methanol (45631)

    Starting at: £71.12

    analytical grade
  27. Methanol for HPLC (45630.01)

    £102.80
    Product Size: 2.5L
  28. NC 2 Nitrocellulose Membrane (71222.01)

    £93.30
    Product Size: 10Pieces
    Pore size 0.2 µm, format 80 x 85 mm
  29. NC 2 Nitrocellulose Membrane, white (71224.01)

    £321.39
    Product Size: 1ROLL
    Pore size 0.2 µm, format: 30 cm x 3 m
  30. NC 2 Nitrocellulose Membrane, white (71223.01)

    £152.43
    Product Size: 5SHEETS
    Pore size 0.2 µm, format: 20 x 20 cm
  31. NC 2 Supported Nitrocellulose Membrane (71227.01)

    £82.24
    Product Size: 10 PIECES
    Pore size 0.2 µm, format 80 x 85 mm
  32. NC 2 Supported Nitrocellulose Membrane (71226.01)

    £376.86
    Product Size: 1 ROLL
    Pore size 0.2 µm, format 30 cm x 3 m
  33. NC 45 Nitrocellulose Membrane, plain, white (42516.01)

    £142.93
    Product Size: 10SHEETS
    Pore size 0.45 µm, format 88 mm x 88 mm
  34. NC 45 Nitrocellulose Membrane, plain, white (71208.01)

    £325.62
    Product Size: 1ROLL
    Pore size 0.45 µm, format 30 cm x 3 m
  35. NC 45 Supported Nitrocellulose Membrane (71225.01)

    £351.52
    Product Size: 1 ROLL
    Pore size 0.45 µm, format 30 cm x 3 m
  36. Nitro Blue tetrazolium chloride (NBT) (30550)

    Starting at: £26.40

    analytical grade
  37. PBST Buffer (10x) (42597.01)

    £66.53
    Product Size: 1L
    sterile
  38. PVDF 0.2 transfer membrane (42515.01)

    £308.35
    Product Size: 1ROLL
    Pore size 0.2 µm, format: 30 cm x 3 m
  39. PVDF0.45 transfer membrane (42514.01)

    £308.35
    Product Size: 1ROLL
    Pore size 0.45 µm, format: 30 cm x 3 m
  40. Semi-dry Blotting Buffer Kit (42559.01)

    £112.99
    Product Size: 3x500ML
    (for Western Blotting)
  41. SERVA VisiBlot Standard I (39260.01)

    £241.82
    Product Size: 500µL
  42. SERVAColor BCIP/NBT Blot Solution (15245.01)

    £163.68
    Product Size: 250ML
  43. SERVALight EosUltra (42586)

    Starting at: £100.32

    CL HRP WB Substrate Kit
  44. SERVALight Helios (42587)

    Starting at: £103.49

    CL HRP WB Substrate Kit
  45. SERVALight Polaris (42584)

    Starting at: £76.03

    CL HRP WB Substrate Kit
  46. SERVALight Vega (42588)

    Starting at: £43.30

    CL HRP WB Substrate Kit