The proprietary RalA G-LISA™ Activation Assaythat is faster, easier and more precise than traditional pull-down methods to measure RalA Activation.
- Specific for RalA
- Colorimetric Based
- Fast Results
- Linear from 0.5 to 5 ng
- Flexible format
General Information
The RalA G-LISA™ kit contains a Ral-GTP-binding protein linked to the wells of a 96 well plate. Active GTP-bound Ral is captured by this protein while inactive GDP-bound Ral is removed following washes. The active RalA bound to the wells is detected with a RalA specific antibody. The degree of RalA activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.
Kit Contents - Enough reagents for 96 assays.
- 96 individual Ral-GTP binding wells
- Anti-RalA monoclonal antibody
- Secondary Antibody - HRP
- RalA control protein
- Ral Binding Buffer
- Cell Lysis Buffer
- Wash buffer
- Antigen Presenting Buffer
- Antibody Dilution Buffer
- HRP Detection and Stop Reagents
- Precision Red™Advanced Protein Assay (Cat. # ADV02)
- Protease Inhibitor Cocktail (Cat. # PIC02)
