QuantiFluo™ Protein Assay Kit

• Fast and sensitive. Assay is completed within a few minutes. Linear detection range of 0.05 - 200 μg/mL BSA.

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

The protein or peptide is known as the "building blocks of life" and is one of the most important macromolecules in life science. Protein determination is a very common practice. Simple, direct and automation-ready procedures for measuring protein or peptide concentration are very desirable. BioAssay Systems’ QuantiFluo™ protein assay kit is based on an improved o-phthalaldehyde method. This reagent reacts with primary amines in protein or peptide and forms a blue fluorescent product, allowing detection of nanograms of proteins. The fluorescence intensity (λex/em = 360/450nm) is proportional to the protein concentration in the sample.