EnzyChrom™ Lactulose Assay Kit

• Fast and sensitive. Use of 40 μL sample. Linear detection range 3 to 300 μM lactulose in 96-well plate assay.

• Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.

• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

LACTULOSE (C12H22O11) is a synthetic sugar that is non-digestible by the gut. It is used to treat constipation and hyperammonemia. It may also be used for intestinal permeability test for leaky gut. Lactulose can be found in milk-based products. Raw milk normally contains extremely low or no level of lactulose. However, the lactulose level in milk will increase after heat-treated.  BioAssay Systems’ lactulose assay kit is based on β-galactosidase catalyzed oxidation of lactulose, which generates D-fructose and D-galactose. The generated D-fructose reacts with the specific reagent to form a colored product whose color intensity, measured at 565 nm, is proportional to the lactulose concentration in the sample.