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Collagenase NB 8 Broad RangeCollagenase NB 8 Broad Range
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Collagenase NB 8 Broad Range

For Cell Isolation of Rodent Pancreas and Tumors

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Collagenase NB 8 Broad Range is a chromatographically purified enzyme resulting in a high collagenolytic activity. This product has an increased collagenase activity compared to Collagenase NB 4 Standard Grade, Collagenase NB 4G Proved Grade and Collagenase NB 7D Gentle Grade. The additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as the endotoxin level are reduced. Collagenase NB 8 is suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes.
Cat# Size Price Qty Buy
S1745601 250 mg
£190.08
S1745602 1 g
£547.01

Additional Information

Property Value or Rating
Manufacturer Nordmark Biochemicals
Storage +2 °C to +8 °C
References

References and Definitions

Unit definition: 
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

Related Documents

References and Definitions

Unit definition: 
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

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