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Figure 1. Comparison of BlastR lysis buffer to alternative lysis
buffers. A431 cells were lysed with BlastR, RIPA, mPER, IP lysis, Denaturing(1% SDS), and Laemmli lysis buffers. All denaturing lysates had genomic DNA removed using BlastR filter. Isolation of proteins from the membrane,cytoplasmic, mitochondrial, and nuclear markers were determined using antibodies against the respective compartment marker proteins.
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Figure 2. BlastR lysis filter is effective at removing genomic DNA. (A) A431 cells were lysed with a denaturing lysis buffer. Genomic DNA was removed or sheared with BlastR filter, syringe needle or soni-cation for 5, 10, 20, and 30 seconds. 2% of lysate was analyzed by ethidi-um bromide, agarose gel electrophoresis. (B) Lysate from A431 cells lysed with a denaturing buffer was either unfiltered or filter with the BlastR filter. Sample were separated with SDS-PAGE and visualized using Coomassie stain. (C) Duplicate samples from B were separated by SDS-PAGE, trans-ferred to PVDE, and EGFR protein was examined using an EGFR anti-
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Figure 3. BlastR lysis buffer characteristics. (A) A431 cells were lysed with BlastR, RIPA, mPER, IP lysis, Denaturing (1% SDS), and Laemmli lysis buffers. All denaturing lysates had genomic DNA removed using the BlastR filter. Coomassie stain was performed to obtain a protein isolation profile with these buffers. (B) Protein quantitation of RIPA, BlastR, 1% SDS, and Laemmli was performed using a standard colorimetric assay (ADV02). A titration of 5, 10, and 20 µL was performed to determine the
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