Food/Beverage Analysis

For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 1.6 pmoles of phosphate and useful range between 0.02 µM and 40 µM phosphate. Fast and convenient: homogeneous "mix-and-measure" assay allows quantitation of free phosphate within 20 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm (malachite green). Samples: Biological, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 2500 tests. Detection limit: 0.02 µM.

For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.

For quantitative determination of D-lactate (D-lactic acid) using the PiCO Explorer handheld absorbance reader.

For quantitative determination of free amino nitrogen using the PiCO Explorer handheld absorbance reader.

For quantitative determination of histamine using the PiCO Explorer handheld absorbance reader.

For quantitative determination of L-Glutamate using the PiCO Explorer handheld absorbance reader.

For quantitative determination of L-lactate (L-lactic acid) using the PiCO Explorer handheld absorbance reader.

For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.

For quantitative determination of calcium ion Ca²⁺ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).

For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl^- in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD610nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.7 mg/dL (0.2 mM).

For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism. Key Features: Sensitive and accurate. Linear detection range 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM) copper in 96-well plate assay. Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Method: OD359nm. Samples: Biological, environment, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 7 µg/dL (1.0 µM).

For quantitative determination of ethanol and alcohol metabolism. Key Features: Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay. Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD580nm (Chemical). Samples: Alcoholic beverages etc. Species: All. Procedure: Assay takes 10 min. Kit size: 500 tests. Detection limit: 0.04%.

For quantitative determination of ferric reduction antioxidant potential (FRAP). Key Features: Sensitive and accurate. Linear detection range: 0.5 to 180 µM Fe³⁺ reduction potential in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Method: OD590nm. Samples: Plant extracts, foods, vitamins, supplements, and biological samples such as serum, plasma, and urine. Species: All. Procedure: Assay takes 40 min. Kit size: 250 tests. Detection limit: 0.5 µM.

For quantitative determination of free amino nitrogen in foods and beverages. Key Features: Fast and sensitive. Linear detection range (5 µL sample): 0.2 to 10 mM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD575nm. Samples: Food and Beverages. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.

For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples. Method: OD630nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.7 mg/dL (39 µM).

For quantitative determination of iron ions Fe³⁺ and/or Fe²⁺ and evaluation of drug effects on iron metabolism. Key Features: Sensitive and accurate. Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Biological (e.g. serum) and environmental samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 27 µg/dL (4.8 µM).

For quantitative determination of Mg²⁺ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).

For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection range 0.6 - 200 µM in 96-well plate. Rapid and reliable. Using an optimized VCl₃ reagent, the time required for reduction of nitrate (NO₃^-) to nitrite (NO₂^-) is reduced to 10 min at 60°C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day. Method: OD540nm. Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.6 µM.

For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism. Key Features: Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals. Method: OD620nm (malachite green). Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM).

For quantitative determination of salicylate in biological samples and beauty products, mouthwash, etc. Key Features: Fast and sensitive. Linear detection range: 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate with 20 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD560nm. Samples: Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 Tests.

For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid. Key Features: Sensitive and accurate. Use as little as 60 µL samples. Linear detection range in 96-well plate: 5 to 1000 µM sialic acid for colorimetric assays and 0.5 to 100 µM for fluorimetric assays. Method: OD549nm, or FL555/585nm. Samples: Serum, plasma, saliva, milk etc. Species: All. Procedure: Assay takes 2.5 hrs. Kit size: 100 tests. Detection limit: OD, FL: 5, 0.5 µM.

For quantitative determination of sulfate ion and evaluation of drug effects on sulfate metabolism. Key Features: Sensitive and accurate. Detection range 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) sulfate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Method: OD600nm. Samples: Serum, urine, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 200 tests. Detection limit: 20 µM.

For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm. Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 1 mg/mL (0.17 mM).

For quantitative determination of zinc ion Zn²⁺ and evaluation of drug effects on zinc metabolism. Key Features: Sensitive and accurate. Uses 50 µL samples. Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed. Method: OD425nm. Samples: Serum, plasma, urine, saliva, food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.78 µg/dL (0.12 µM).

For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µg/mL.

For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological (eg urine) and environ-mental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 200 tests. Detection limit: 12 µM.

For quantitative determination of diamine oxidase and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Serum, Plasma. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

For rapid, direct determination of D-lactic acid concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-180 mg/L (undiluted) D-lactic acid. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

For rapid, direct determination of glucose concentrations in food and beverage samples as well as biological (e.g. urine, blood, etc.) samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-720 mg/L (undiluted) Glucose. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, juice, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

For rapid, direct determination of Histamine concentrations in fish, wine, and other samples. Key Features: Fast and sensitive. Use of 500 mg of fish or 250 µL of sample. Semi-quantitative measurement between 0-200 ppm (undiluted) Histamine. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Seafood, wine, juice, milk and dairy products, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

For rapid, direct determination of L-Glutamate (monosodium glutamate or MSG) concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-300 mg/L (undiluted) L-Glutamate. Convenient. No extra equipment required. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Soups, sauces, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

For rapid, direct determination of L-lactic acid concentrations in food and beverage samples as well as biological samples. Semi-quantitative measurement between 0-360 mg/L (undiluted) L-lactic acid. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

For rapid, direct determination of malic acid concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-500 mg/L (undiluted) malic acid. Convenient. No expensive lab equipment needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

For rapid, direct determination of pyruvate concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 50, 100, or 400 µL sample. Semi-quantitative measurement between 0-131 mg/L (undiluted) Pyruvate. Convenient. No expensive plate or cuvette readers needed. Sample treatment and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, biological samples, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.