Blood/Urine Chemistry Assays

For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.05 to 60 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.05 U/L.

For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological (e.g. plasma, serum, urine, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.4 U/L.

For quantitative determination of arginase activity and screen for its inhibitors. Key Features: Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format. Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD430nm. Samples: Enzyme preparations, serum, plasma, tissue culture etc. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: 1 U/L (0.3 U/L for 2 hr reaction).

For quantitative determination of albumin and evaluation of drug effects on albumin metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Detection range 0.01 - 5 g/dL (1.5 - 750 µM) albumin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. No interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD620nm (BCG). Samples: Serum, plasma, urine, biological preparations. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.01 g/dL.

For quantitative determination of albumin and evaluation of drug effects on albumin metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Detection range 0.3 - 5 g/dL (45 - 750 µM) albumin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD610nm (BCP). Samples: Serum, plasma, urine, biological preparations. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.3 g/dL.

For quantitative determination of bilirubin and evaluation of drug effects on bilirubin metabolism. Key Features: Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD530nm. Samples: Serum. Species: All. Procedure: Assay takes 10 min. Kit size: 180 tests. Detection limit: 0.16 mg/dL.

For quantitative determination of calcium ion Ca²⁺ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).

For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl^- in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD610nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.7 mg/dL (0.2 mM).

For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).

For quantitative determination of ethanol and alcohol metabolism. Key Features: Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay. Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD580nm (Chemical). Samples: Alcoholic beverages etc. Species: All. Procedure: Assay takes 10 min. Kit size: 500 tests. Detection limit: 0.04%.

For quantitative and direct determination of formaldehyde concentrations in biological, food and environmental samples. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 1.5 µM (45 ppb) formaldehyde can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL370/470nm. Samples: Biological, food, beverage, environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 1.5 µM (45 ppb).

For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples. Method: OD630nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.7 mg/dL (39 µM).

For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 200 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, tissue and culture media). Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 100 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

For quantitative determination of glutamate dehydrogenase activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell, tissue, agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 U/L.

For quantitative determination of heme and evaluation of drug effects on heme metabolism. Key Features: Sensitive and accurate. Linear detection range 0.6 - 125 µM heme in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents are non-toxic. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.6 µM.

For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism. Key Features: Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.9 mg/dL.

For quantitative determination of indican (indoxyl sulfate) in urine samples.

For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 100 U/L for 30 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Plasma, serum,tissue and culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 U/L.

For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators. Key Features: High sensitivity and wide linear range. Use 3 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 200 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of LDH activity within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD565nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 U/L.

For quantitative determination of lipase enzyme activity. Key Features: Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay. Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day. Method: OD412nm. Samples: Serum, plasma, saliva, urine etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 40 U/L.

For quantitative determination of Mg²⁺ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).

For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism. Key Features: Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals. Method: OD620nm (malachite green). Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM).

For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR). Key Features: Sensitive and accurate. Use 20 µL samples. Linear detection range in 96-well plate: 1 - 20 mg/dL Protein and 1 - 150 mg/dL Creatinine. Fast and convenient. No sample pre-treatment is needed. Simple 10- minute "add-incubate-read" procedure. High-throughput adaptable. The procedure can be readily automated for processing thousands of samples per day. Method: OD600 and OD530nm. Samples: Urine samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 1 mg/dL Protein and 1 mg/dL Creatinine.

For quantitative determination of salicylate in biological samples and beauty products, mouthwash, etc. Key Features: Fast and sensitive. Linear detection range: 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate with 20 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD560nm. Samples: Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 Tests.

For quantitative determination of sulfate ion and evaluation of drug effects on sulfate metabolism. Key Features: Sensitive and accurate. Detection range 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) sulfate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Method: OD600nm. Samples: Serum, urine, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 200 tests. Detection limit: 20 µM.

For quantitative determination of total protein.

For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm. Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 1 mg/mL (0.17 mM).

For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 mg/dL (13 µM).

For quantitative determination of hemoglobin (Hb) in whole blood. Key Features: Safety. Non-hazardous. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvette. High sensitivity. As low as 5 µL sample is used. Ideal when samples are limited such as mice blood samples. Method: OD570nm. Samples: Whole blood samples. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.2 g/dL.

For quantitative determination of zinc ion Zn²⁺ and evaluation of drug effects on zinc metabolism. Key Features: Sensitive and accurate. Uses 50 µL samples. Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed. Method: OD425nm. Samples: Serum, plasma, urine, saliva, food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.78 µg/dL (0.12 µM).

For quantitative determination of α-glucosidase activity and evaluation of drug effects on its metabolism. Key Features: High sensitivity and wide linear range. Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 U/L.

For quantitative determination of α-L-Fucosidase activity in biological samples. Key Features: High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L. Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Can be readily automated to measure thousands of samples per day. Method: OD405nm. Samples: Plasma, serum, tissue, cell lysate, etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 U/L.

For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism. Key Features: High sensitivity and wide linear range. Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 U/L.

For quantitatve determination of β-N-Acetylglucosaminidase activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 50 U/L for a 30 minute reaction at 37°C. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Urine, serum, plasma, cell lysate, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.2 U/L.

For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.