Anti-actin antibody (rabbit polyAb) + controlAnti-actin antibody (rabbit polyAb) + control
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Anti-actin antibody (rabbit polyAb) + control

This antibody is recommended for detection of actin in human, mouse, rat, xenopus, and bovine extracts. Rabbit polyclonal antibody against actin protein. Actin is the major protein of the microfilament cytoskeletal system and is a key protein in various cell motility processes. The immunogen used for antibody production was a peptide consisting of the 11 C-terminal amino acids of actin. Human platelet extract (Cat. # EXT01) is included as a positive control for Western blot analysis. A characteristic actin band at 43 kDa is identified on Western blots. Antiactin antibody is supplied as a lyophilized white powder.

Cat# Size Price Qty Buy
AAN01-A 1 x 100 µg
£202.80
AAN01-B 3 x 100 µg
£487.50

Additional Information

Property Value or Rating
Manufacturer Cytoskeleton, Inc
Storage 4°C
References
Author(s): Bi Q. et al.
(
See PubMed article)

Cell Death Dis. 2015; 6, e1767.
"A cellular screen identifies ponatinib and pazopanib as inhibitors of necroptosis"
Author(s): Fauster A. et al.
(See PubMed article)

Mol. Psychiatry. 2015; doi: 10.1038/mp.2014.172.
"Identification and functional characterization of rare SHANK2 variants in schizophrenia"
Author(s): Peykov S. et al.
(See PubMed article)

Biochem. Biophys. Res. Commun. 2014; 454, 335–340.
"Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines"
Author(s): Kacsinta A.D. et al.
(See PubMed article)

Anti-actin antibody (Cat. # AAN01) was used as a Western blot loading control to confirm that differences in Erk activation were not due to differential protein loading in lysates from human mesenchymal stem cells.
Mol. Biol. Cell 2005; 16, 881-890.
"Laminin-5 induces osteogenic gene expression in human mesenchymal stem cells through an ERK-dependent pathway."
Author(s): Klees, R. F., Salasznyk, R. M., Kingsley, K., Williams, W. A., Boskey, A. and Plopper, G. E.
(See PubMed article)

Anti-actin antibody (Cat. # AAN01) was used in the study of muscle force regulation to measure changes in G and F-actin ratios as detected by Western blotting in swine carotid artery tissues.
Am. J. Physiol. 2005; 288, C633-639.
"Heat shock protein 20-mediated force suppression in forskolin-relaxed swine carotid artery."
Author(s): Meeks, M. K., Ripley, M. L., Jin, Z. and Rembold, C. M.
(See PubMed article)

Anti-actin antibody (Cat.# AAN01) was used in immunocytochemistry and Western blotting experiments using mouse pancreatic acinar cells to study the functional link between inositol (1,4,5)-trisphosphate receptors and actin in controlling calcium signaling.
J. Cell Sci. 2005; 118, 971-980.
"Inositol 1,4,5-trisphosphate receptor links to filamentous actin are important for generating local Ca2+ signals in pancreatic acinar cells."
Author(s): Turvey, M. R., Fogarty, K. E. and Thorn, P.
(See PubMed article)

J. Biol. Chem. 2004; 279, 14039-14048.
"N terminus is essential for tropomyosin functions: N-terminal modification disrupts stress fiber organization and abolishes anti-oncogenic effects of tropomyosin-1."
Author(s): Bharadwaj, S., Hitchcock-DeGregori, S., Thorburn, A. and Prasad, G. L.
(See PubMed article)

J. Biol. Chem. 2004; 279, 39705-39709.
"Protective effect of phosphatidylinositol 4,5-bisphosphate against cortical filamentous actin loss and insulin resistance induced by sustained exposure of 3T3-L1 adipocytes to insulin."
Author(s): Chen, G., Raman, P., Bhonagiri, P., Strawbridge, A. B., Pattar, G. R. and Elmendorf, J. S.
(See PubMed article)

Am. J. Physiol. 2004; 286, G645-652.
"Escherichia coli LPS induces heat shock protein 25 in intestinal epithelial cells through MAP kinase activation."
Author(s): Kojima, K., Musch, M. W., Ropeleski, M. J., Boone, D. L., Ma, A. and Chang, E. B.
(See PubMed article)

J. Cell Biol. 2004; 167, 545-554.
"Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility."
Author(s): Strachan, L. R. and Condic, M. L.
(See PubMed article)

Am. J. Physiol. 2003; 285, L643-653.
"Aberrant lung structure, composition, and function in a murine model of Hermansky-Pudlak syndrome."
Author(s): Lyerla, T. A., Rusiniak, M. E., Borchers, M., Jahreis, G., Tan, J., Ohtake, P., Novak, E. K. and Swank, R. T.
(See PubMed article)

Mol. Biol. Cell 2003; 14, 2492-2507.
"Caenorhabditis elegans UNC-98, a C2H2 Zn finger protein, is a novel partner of UNC-97/PINCH in muscle adhesion complexes."
Author(s): Mercer, K. B., Flaherty, D. B., Miller, R. K., Qadota, H., Tinley, T. L., Moerman, D. G. and Benian, G. M.
(See PubMed article)

J. Cell Sci. 2003; 116, 2073-2085.
"Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent processes in Caenorhabditis elegans."
Author(s): Ono, K., Parast, M., Alberico, C., Benian, G. M. and Ono, S.
(See PubMed article)

" title="

Clin. Exp. Metastasis. 2015; 32, 301-311.
"MTBP inhibits migration and metastasis of hepatocellular carcinoma"
Author(s): Bi Q. et al.
(See PubMed article)

Cell Death Dis. 2015; 6, e1767.
"A cellular screen identifies ponatinib and pazopanib as inhibitors of necroptosis"
Author(s): Fauster A. et al.
(See PubMed article)

Mol. Psychiatry. 2015; doi: 10.1038/mp.2014.172.
"Identification and functional characterization of rare SHANK2 variants in schizophrenia"
Author(s): Peykov S. et al.
(See PubMed article)

Biochem. Biophys. Res. Commun. 2014; 454, 335–340.
"Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines"
Author(s): Kacsinta A.D. et al.
(See PubMed article)

Anti-actin antibody (Cat. # AAN01) was used as a Western blot loading control to confirm that differences in Erk activation were not due to differential protein loading in lysates from human mesenchymal stem cells.
Mol. Biol. Cell 2005; 16, 881-890.
"Laminin-5 induces osteogenic gene expression in human mesenchymal stem cells through an ERK-dependent pathway."
Author(s): Klees, R. F., Salasznyk, R. M., Kingsley, K., Williams, W. A., Boskey, A. and Plopper, G. E.
(See PubMed article)

Anti-actin antibody (Cat. # AAN01) was used in the study of muscle force regulation to measure changes in G and F-actin ratios as detected by Western blotting in swine carotid artery tissues.
Am. J. Physiol. 2005; 288, C633-639.
"Heat shock protein 20-mediated force suppression in forskolin-relaxed swine carotid artery."
Author(s): Meeks, M. K., Ripley, M. L., Jin, Z. and Rembold, C. M.
(See PubMed article)

Anti-actin antibody (Cat.# AAN01) was used in immunocytochemistry and Western blotting experiments using mouse pancreatic acinar cells to study the functional link between inositol (1,4,5)-trisphosphate receptors and actin in controlling calcium signaling.
J. Cell Sci. 2005; 118, 971-980.
"Inositol 1,4,5-trisphosphate receptor links to filamentous actin are important for generating local Ca2+ signals in pancreatic acinar cells."
Author(s): Turvey, M. R., Fogarty, K. E. and Thorn, P.
(See PubMed article)

J. Biol. Chem. 2004; 279, 14039-14048.
"N terminus is essential for tropomyosin functions: N-terminal modification disrupts stress fiber organization and abolishes anti-oncogenic effects of tropomyosin-1."
Author(s): Bharadwaj, S., Hitchcock-DeGregori, S., Thorburn, A. and Prasad, G. L.
(See PubMed article)

J. Biol. Chem. 2004; 279, 39705-39709.
"Protective effect of phosphatidylinositol 4,5-bisphosphate against cortical filamentous actin loss and insulin resistance induced by sustained exposure of 3T3-L1 adipocytes to insulin."
Author(s): Chen, G., Raman, P., Bhonagiri, P., Strawbridge, A. B., Pattar, G. R. and Elmendorf, J. S.
(See PubMed article)

Am. J. Physiol. 2004; 286, G645-652.
"Escherichia coli LPS induces heat shock protein 25 in intestinal epithelial cells through MAP kinase activation."
Author(s): Kojima, K., Musch, M. W., Ropeleski, M. J., Boone, D. L., Ma, A. and Chang, E. B.
(See PubMed article)

J. Cell Biol. 2004; 167, 545-554.
"Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility."
Author(s): Strachan, L. R. and Condic, M. L.
(See PubMed article)

Am. J. Physiol. 2003; 285, L643-653.
"Aberrant lung structure, composition, and function in a murine model of Hermansky-Pudlak syndrome."
Author(s): Lyerla, T. A., Rusiniak, M. E., Borchers, M., Jahreis, G., Tan, J., Ohtake, P., Novak, E. K. and Swank, R. T.
(See PubMed article)

Mol. Biol. Cell 2003; 14, 2492-2507.
"Caenorhabditis elegans UNC-98, a C2H2 Zn finger protein, is a novel partner of UNC-97/PINCH in muscle adhesion complexes."
Author(s): Mercer, K. B., Flaherty, D. B., Miller, R. K., Qadota, H., Tinley, T. L., Moerman, D. G. and Benian, G. M.
(See PubMed article)

J. Cell Sci. 2003; 116, 2073-2085.
"Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent processes in Caenorhabditis elegans."
Author(s): Ono, K., Parast, M., Alberico, C., Benian, G. M. and Ono, S.
(See PubMed article)

" target="_blank">

Clin. Exp. Metastasis. 2015; 32, 301-311.
"MTBP inhibits migration and metastasis of hepatocellular carcinoma"
Author(s): Bi Q. et al.
(See PubMed article)

Cell Death Dis. 2015; 6, e1767.
"A cellular screen identifies ponatinib and pazopanib as inhibitors of necroptosis"
Author(s): Fauster A. et al.
(See PubMed article)

Mol. Psychiatry. 2015; doi: 10.1038/mp.2014.172.
"Identification and functional characterization of rare SHANK2 variants in schizophrenia"
Author(s): Peykov S. et al.
(See PubMed article)

Biochem. Biophys. Res. Commun. 2014; 454, 335–340.
"Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines"
Author(s): Kacsinta A.D. et al.
(See PubMed article)

Anti-actin antibody (Cat. # AAN01) was used as a Western blot loading control to confirm that differences in Erk activation were not due to differential protein loading in lysates from human mesenchymal stem cells.
Mol. Biol. Cell 2005; 16, 881-890.
"Laminin-5 induces osteogenic gene expression in human mesenchymal stem cells through an ERK-dependent pathway."
Author(s): Klees, R. F., Salasznyk, R. M., Kingsley, K., Williams, W. A., Boskey, A. and Plopper, G. E.
(See PubMed article)

Anti-actin antibody (Cat. # AAN01) was used in the study of muscle force regulation to measure changes in G and F-actin ratios as detected by Western blotting in swine carotid artery tissues.
Am. J. Physiol. 2005; 288, C633-639.
"Heat shock protein 20-mediated force suppression in forskolin-relaxed swine carotid artery."
Author(s): Meeks, M. K., Ripley, M. L., Jin, Z. and Rembold, C. M.
(See PubMed article)

Anti-actin antibody (Cat.# AAN01) was used in immunocytochemistry and Western blotting experiments using mouse pancreatic acinar cells to study the functional link between inositol (1,4,5)-trisphosphate receptors and actin in controlling calcium signaling.
J. Cell Sci. 2005; 118, 971-980.
"Inositol 1,4,5-trisphosphate receptor links to filamentous actin are important for generating local Ca2+ signals in pancreatic acinar cells."
Author(s): Turvey, M. R., Fogarty, K. E. and Thorn, P.
(See PubMed article)

J. Biol. Chem. 2004; 279, 14039-14048.
"N terminus is essential for tropomyosin functions: N-terminal modification disrupts stress fiber organization and abolishes anti-oncogenic effects of tropomyosin-1."
Author(s): Bharadwaj, S., Hitchcock-DeGregori, S., Thorburn, A. and Prasad, G. L.
(See PubMed article)

J. Biol. Chem. 2004; 279, 39705-39709.
"Protective effect of phosphatidylinositol 4,5-bisphosphate against cortical filamentous actin loss and insulin resistance induced by sustained exposure of 3T3-L1 adipocytes to insulin."
Author(s): Chen, G., Raman, P., Bhonagiri, P., Strawbridge, A. B., Pattar, G. R. and Elmendorf, J. S.
(See PubMed article)

Am. J. Physiol. 2004; 286, G645-652.
"Escherichia coli LPS induces heat shock protein 25 in intestinal epithelial cells through MAP kinase activation."
Author(s): Kojima, K., Musch, M. W., Ropeleski, M. J., Boone, D. L., Ma, A. and Chang, E. B.
(See PubMed article)

J. Cell Biol. 2004; 167, 545-554.
"Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility."
Author(s): Strachan, L. R. and Condic, M. L.
(See PubMed article)

Am. J. Physiol. 2003; 285, L643-653.
"Aberrant lung structure, composition, and function in a murine model of Hermansky-Pudlak syndrome."
Author(s): Lyerla, T. A., Rusiniak, M. E., Borchers, M., Jahreis, G., Tan, J., Ohtake, P., Novak, E. K. and Swank, R. T.
(See PubMed article)

Mol. Biol. Cell 2003; 14, 2492-2507.
"Caenorhabditis elegans UNC-98, a C2H2 Zn finger protein, is a novel partner of UNC-97/PINCH in muscle adhesion complexes."
Author(s): Mercer, K. B., Flaherty, D. B., Miller, R. K., Qadota, H., Tinley, T. L., Moerman, D. G. and Benian, G. M.
(See PubMed article)

J. Cell Sci. 2003; 116, 2073-2085.
"Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent processes in Caenorhabditis elegans."
Author(s): Ono, K., Parast, M., Alberico, C., Benian, G. M. and Ono, S.
(See PubMed article)

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