SERVA Electrophoresis GmbH

http://www.serva.de/

Electrophoresis, Collagenases, Biochemicals and Bioseparation Reagents

Featuring:

• Protein Electrophoresis (ampholytes and precast gels)
• Nucleic Acid Electrophoresis
• Blotting
• Staining Reagents
• Reagents for use in Electrophoresis
• Extraction and Concentration Tools
• Electrophoresis Equipment
• Proteomics
• Collagenases and Related Enzymes
• Biochemicals
• Bioseparation Reagents

Protein Electrophoresis

     Isoelectric Focusing

• SERVALYT™ Carrier Ampholytes | UK Prices
  Available as wide range, narrow range and ultra-narrow range, high resolution due to multimeric composition. Fast staining and destaining times.
  
• SERVALYT™ PRECOTES™ | UK Prices
  Precast gels for Horizontal IEF, general purpose, ready-to-use, with selected pH ranges and offered with thin and ultrathin gel layers.
  
• SERVALYT™ PRECOTES™ Kits | UK Prices
  IEF Starter Kit plus special applications kits for IEF of Cerebrospinal Fluid, Haptoglobin, Transferrin and Phosphogluomutae. Easy-to-start, ready-to-use.
  
• SERVALYT™ PreNets™ | UK Prices
  ready-to-use precast gels with NetFix™ ideal for subsequent blotting transfer.
  
• Blank PRECOTES™ | UK Prices
  Multifunctional precast gels for any pH range, to be equilibrated in ampholyte of choice, also suited for IEF in the presence of urea.
  
• Blank PreNets™ | UK Prices
  Multifunctional precast gels with NetFix™, for any pH range of choice and blotting transfer.

Accessories for Horizontal PAGE

• Accessories for IEF
  
• Accessories | UK Prices
  Adhesive tape, Anode fluid, Applicator strips, BlueSlick spray, Cathode fluid, Electrode wicks, Gaskets, PGM Buffers, Spacer strips
  

SDS and Native PAGE

• Precast Horizontal Gels | UK Prices
  Precast gels for SDS PAGE, improved system resolving proteins from 10kDa to 200kDa and facilitating blotting.
  
• SERVAGel™ Precast Vertical Gels | UK Prices
  Precast gels in cassettes for vertical electrophoresis of proteins. For separation of proteins in the presence of (SDS PAGE) or absence (native PAGE) of SDS.
• SERVA IPG BlueStrips | UK Prices
  Dried gel strips with immobilised pH gradient for ID- and 2D-gel electrophoresis of proteins
  
• Rehydration Tray for SERVA IPG Strip | UK Prices

     Gel Preparation

• GEL-FIX™ | UK Prices
  Supporting film for casting of gels
  
• NetFix™ | UK Prices
  Inert supporting film for gel layers
  
• Acrylamide powders and solutions | UK Prices
  
• Agaroses for IEF | UK Prices

     Protein Markers

• Protein Markers for IEF | UK Prices
  Ready-to-use & lyophilised, applicable to all vertical/horizontal IEF gels.
  
• Protein Markers for SDS PAGE | UK Prices
  
• Protein Markers for Native PAGE | UK Prices
  
• Proteome Marker | UK Prices

Nucleic Acid Electrophoresis

     Nucleic Acid Electrophoresis Reagents

• DNA Molecular Weight Markers | UK Prices
  
• Submarine Electrophoresis: Agarose Media | UK Prices

Blotting

     Blotting Reagents

• Transfer Buffers | UK Prices

Staining Reagents

SERVA offers a broad and comprehensive range of dyes and stains for protein detection available in powder, tablet and ready-to-use solutions.

Dyes and Stains | UK Prices

• Acridine Orange, Amido Black 10B, Bromophenol Blue, Coomassie Brilliant Blue (G250 & R250),
• Stains-all, Ethidium bromide, Fast Green FCF, Fast Red TR-salt,
• Methylene Blue, Ponceau S, Pyronin Y
• Serva Blue G, Serva Blue R, Serva Blue W
• Serva Silver Staining Kits – kits for easy staining of proteins after native PAGE or SDS PAGE gel electrophoresis.
• Serva Violet 17, Silver nitrate, Toluidine Blue O salt, Xylene cyanol FF

Reagents for Use in Electrophoresis

         Biochemicals, Buffers, Enzymes & Surfactants

• BlueSlick | UK Prices
  non-stick, non-toxic reagent for treatment of glass plates
  
• Reagents from A-Z | (Please enquire for UK Prices)
  
• SDS Pellets – no stench or irritant dust | UK Prices
  
• Ready-to-use Buffers and Solutions | UK Prices
  
• DNase-S | UK Prices
  For purification of proteins and other biologicals in sample preparation for electrophoresis.

Electrophoresis Equipment

     Horizontal Electrophoresis

• BlueMarine Horizontal Submarine Units | UK Prices
  For separation of nucleic acids in agarose. Rapid screening of PCR fragments, analysis of restriction digests and plasmid preparations, checking in vitro transcripts etc.
  
• BlueHorizon™ Super Cool Flatbed System | UK Prices
  A flatbed system for horizontal applications using precast gels and/or self-cast gels frequently used to perform Isoelectric Focusing (IEF) and SDS PAGE, but also for separation of nucleic acids.
  

     Vertical Electrophoresis

• BlueVertical Mini Slab Gel System | UK Prices
  A dual mini tank with option to operate one or two precast / self-cast gels. SDS-PAGE, IEF and native PAGE can be carried out.

     Electroblotting

• BlueBlot Wet Tank Blotters | UK Prices
  BlueBlot Wet Series for tank blotting of proteins and nucleic acids.
  
• BlueFlash Semi-Dry Blotters | UK Prices
  For fast and efficient Semi-Dry Blotting

     Power Supplies

• BluePower Power Supplies | UK Prices
  A range of power supplies tocover a large variety of separations.

      Accessories

• BlueLink Crosslinker | UK Prices
  For immobilisation of nucleic acids to membranes – cross-linking within seconds.
  
• Transilluminators: UV Tables | UK Prices
  UV Tables with filters in two sizes for wavelengths 254nm or 312nm.
• Rocking Table Kick II | UK Prices
  Large size incubation platform suitable for various incubation and preparation techniques.

Proteomics

     Tools for Proteomics

• Proteome Markers | UK Prices
  A unique and useful tool to calibrate 2D gels, or as an internal LC/MS standard or to be added to protein samples. Available as native blend and as tryptic digest.
  
• SERVA ICPL™ Kit | UK Prices
  for quantitative and qualitative analysis of proteins in proteome studies by mass spectrometry. It is the only method that shows highly accurate and reproducible quantification of proteins and is able to analyse post-translational modifications and protein isoforms.
  
• ProtaQuant Assay Kit | UK Prices
  Reliable, precise and reproducible determinations of protein concentrations in high throughput procedures.
  
• OMX-S | UK Prices
  Multifunctional Sampling Device for MS
  
• Trypsin NB Sequencing Grade | UK Prices
  Highly purified for digestion of proteins prior to mass spectrometry analysis.
  
• SERVA IPG BlueStrips | UK Prices
  Dried gel strips with immobilised pH gradient for ID- and 2D-gel electrophoresis of proteins
  
• Rehydration Tray for SERVA IPG Strips. | UK Prices

Collagenases for Tissue Dissociation

SERVA Collagenase NB qualities from Chlostridum histolyticum are produced by Nordmark the world’s largest manufacturer of collagenases. They are designed for different fields of application. Superb quality and performance are assured by:

• Stringent quality control
• Optimised purification steps
• Accurate analysis of proteolytic enzyme activities
• Manufacturing according to GMP guidelines including process control

     Collagenases from Clostridium histolyticum

• Collagenase NB 1 Premium Grade | UK Prices
  Highly purified for isolation of human pancreatic islet cells of Langerhans in combination with Neutral Protease.
  
• Collagenase NB 4 Standard Grade | UK Prices
  For isolation of various cell types including hepatocytes, cardiomyocytes, pancreatic islet cells, nerve and endothelial cells, chondrocytes, tumor cells and others.
  
• Collagenase NB 4G Proved Grade | UK Prices
  Suitable for isolation of a broad variety of cell types, especially for fibroblasts, adipocytes, hepatocytes (small animals) and frog oocytes. Function tested.
  
• Collagenase NB 5 Sterile Grade | UK Prices
  For applications, as for NB 4 Standard Grade, requiring sterile Collagenase.
  
• Collagenase NB 6 GMP Grade | UK Prices
  For applications, as for NB 4 Standard Grade, which have to meet GMP requirements.
  
• Collagenase NB 8 Broad Range | UK Prices
  For isolation of pancreatic islet cells, cardiomyocytes, hepatocytes, chondrocytes and other from humans or large animals.
  
• Collagenase N Reference Content | UK Prices
  Reference substance for enzyme activity determination according to GMP guidelines.
  
• Collagenase N Reference Identity | UK Prices
  Reference substance for standard controls by SDS-PAGE analysis performed upon receipt of incomoing goods according to GMP guidelines.

      Related Enzymes

• Clostripain NB from Clostridium histolyticum. | UK Prices
  
• Elastase from human neutrophils ca. 18 U/mg lyophilised | UK Prices
  
• Elastase from porcine pancreas min ca. 120 U/mg | UK Prices
  
• Hyaluronidase from bovine testes ca. 1000 U/mg | UK Prices
  
• Neural Protease NB from C. histolyticum. | UK Prices
  
• Trypsin 1:250 from bovine pancreas ca. 4-6 U/mg | UK Prices
  
• Trypsin NB from porcine pancreas | UK Prices
  
• Trypsin NB GMP Grade from porcine pancreas. | UK Prices
  
• Tyypsin NB Sequencing Grade from porcine pancreas. | UK Prices

      Enzyme Inhibitors

• A-Z listing of single protease inhibitors and protease inhibitor mixes for given applications. | (Please enquire for UK Prices)

Biochemicals

     Albumins and Other Proteins

• Albumins and Other Proteins | UK Prices
• Albumins prepared from Bovine Plasma
• Albumins prepared by other methods
• Other Proteins

     Antibiotics

• Antimetabolites | (Please enquire for UK Prices)
• Inhibitors of Bacterial Cell Wall Synthesis | (Please enquire for UK Prices)
  
• Inhibitors of Cell Membrane Functions | (Please enquire for UK Prices)
  
• Inhibitors of Protein Synthesis | (Please enquire for UK Prices)
  
• Inhibitors of Transcription and Replication of Genetic Material | (Please enquire for UK Prices)
  
• Inhibitors / Modifiers of other Cell Functions | (Please enquire for UK Prices)

     Enzymology

• Enzymes - A-Z complete listing | (Please enquire for UK Prices)
  
• Enzyme Inhibitors - A-Z complete listing | (Please enquire for UK Prices)
  
• Enzyme Substrates - A-Z complete listing | (Please enquire for UK Prices)

     Molecular Biology

• Molecular Biology Grade Reagents tested for absence of DNase and RNases | (Please enquire for UK Prices)
  All products are free of contaminating nucleases (DNase, RNase)

Bioseparation Reagents

     Dialysis Tubing

• SERVA VISKING® Dialysis Tubing | (Please enquire for UK Prices)
• SERVAPOR® Dialysis Tubing | (Please enquire for UK Prices)
• MEMBRA-CEL™ Dialysis Tubing | (Please enquire for UK Prices)
• SPECTRA/POR Dialysis Tubings | (Please enquire for UK Prices)

     Ion Exchangers

• DOWEX® Ion Exchange Resins | (Please enquire for UK Prices)
  
• SERVACEL® Cellulose Ion Exchangers | (Please enquire for UK Prices)
  
• SERDOLIT® Chelating Resins | (Please enquire for UK Prices)
  
• SERDOLIT® Ion Exchange Resins – analytical grade quality | (Please enquire for UK Prices)
  
• SERDOLIT® Polymeric Adsorbent Resins | (Please enquire for UK Prices)