Cytoskeleton Inc

http://www.cytoskeleton.com/

Cytoskeleton / Signal Transduction Reagents, Assays and Drug Discovery Tools

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Featuring:

• Cytoskeletal Proteins (Actin, Tubulin, Kinesin, Intermediate Filament Proteins & Kits)
• Small G-proteins and Signal Transduction (G-protein, Modulator/Effector Proteins & Kits)
• CellVision Prepared Cells on Coverslips
• Biochem Kits™
• Drug Discovery / High Throughput CytoDYNAMIX Screens™

Cytoskeletal Proteins

Cytoskeleton Inc offers the widest range of Cytoskeletal Proteins including Actin proteins, Tubulin proteins and Kinesin motor proteins with associated assay kits, antibodies and reagents.

Actin Proteins

Purified Actin Proteins

• Cardiac muscle protein (purified) | UK Prices
• Non-muscle actin (>99% pure) | UK Prices
• Pre-formed Actin Filaments | UK Prices
• Non-muscle actin (>95% pure) | UK Prices
• Skeletal muscle actin (>99% pure) | UK Prices
• Skeletal muscle actin (>95% pure) | UK Prices
• Smooth muscle actin (purified) | UK Prices

Labeled Actin Proteins

• Biotin skeletal musle actin | UK Prices
• Biotin non muscle actin | UK Prices
• Pyrene actin | UK Prices
• Rhodamine skeletal muscle actin | UK Prices
• Rhodamine actin, non-muscle | UK Prices

Actin Binding Proteins

• α-Actinin protein | UK Prices
• Arp2/3 protein complex | UK Prices
• Cofilin protein | UK Prices
• Gelsolin protein | UK Prices
• Myosin Cardiac Protein | UK Prices
• Myosin: Heavy meromysin | UK Prices
• Myosin II protein | UK Prices
• Profilin protein | UK Prices
• WASP VCA domain protein, GST tagged | UK Prices

Actin & Actin Binding Antibodies

• Actin antibody, rabbit polyclonal | UK Prices
• Actin-ß-isotype, mouse monoclonal | UK Prices
• Arp3, sheep polyclonal | UK Prices
• Cofilin, rabbit polyclonal | UK Prices
• Fibrillarin, mouse monoclonal | UK Prices
• Sheep IgG HRP (sec.antibody) | UK Prices
• Sheep IgG Rhodamine labeled (sec. antibody) | UK Prices

Actin Biochem Kits™

• Actin binding protein spin-down assay, skeletal muscle actin | UK Prices
• Actin binding protein spin-down assay, non-muscle actin | UK Prices
• Actin filament kit, skeletal muscle actin | UK Prices
• Actin polymerization assay | UK Prices
• G-actin: F-actin in vivo assay | UK Prices
• Cytoskeleton isolation kit | UK Prices
• F-actin visualisation Kit | UK Prices

Actin Product Buffers & Reagents

• Cytoskelfix™ Cell Fixative | UK Prices
• Actin polymerisation buffer | UK Prices
• ATP (100 mM stock solution) | UK Prices
• General Actin Buffer | UK Prices
• Phalloidin (rhodamine labeled) | UK Prices

Tubulin Proteins

Bovine Tubulins

• Pre-formed Microtubules | UK Prices
• Tubulin (>99% pure, minus glycerol, lyophilised) | UK Prices
• Tubulin (>99% pure, minus glycerol, frozen liquid) | UK Prices
• Tubulin (>99% pure, plus glycerol, frozen liquid) | UK Prices
• Tubulin (>97% pure, for HTS applications, lyophilised) | UK Prices
• Tubulin, MAP rich (lyophilised) | UK Prices

Porcine Tubulins

• Tubulin (>99% pure, minus glycerol, lyophilised) | UK Prices
• Tubulin, MAP rich (lyophilised) | UK Prices

Cancer Cell Tubulins

• HeLa cell tubulin | UK Prices
• MCF-7 cell tubulin | UK Prices

Bacterial tubulin homologs

• FtsZ protein | UK Prices

Fungal Tubulins

• Agaricus bisporus tubulin | UK Prices

Labeled Tubulins

• Biotin tubulin (bovine, lyophilised) | UK Prices
• Biotin tubulin (HeLa cell, lyophilised) | UK Prices
• Biotin tubulin (MCF-7, cell, lyophilised) | UK Prices
• Fluorescein tubulin, minus glycerol (frozen liquid) | UK Prices
• Rhodamine tubulin (lyophilised) | UK Prices
• Fluorescein tubulin, X-rhodamine, minus glycerol (lyophilized) | UK Prices
• Rhodamine tubulin, minus glycerol (frozen liquid) | UK Prices
• Rhodamine tubulin, plus glycerol (frozen liquid) | UK Prices

Tubulin Antibodies

• Tubulin Antibody, (α and ß) whole protein, pan specific, sheep polyclonal | UK Prices
• Tubulin Antibody, ß isotype, mouse monoclonal | UK Prices

Tubulin Biochem Kits™

• Cytoskeleton isolation Kit | UK Prices
• Fluorescent microtubules Kit | UK Prices
• Microtubule binding protein spin-down assay | UK Prices
• Microtubule : tubulin in vivo assay | UK Prices
• Microtubules / tubulin Biochem Kit™ | UK Prices
• Microtubule stabilisation / destabilisation assay | UK Prices
• Tubulin polymerisation assay, OD based, >97% pure tubulin | UK Prices
• Tubulin polymerisation assay, OD based, >99% pure tubulin | UK Prices
• Tubulin polymerisation assay, fluorescence based | UK Prices

HTS Assays for Tubulin and Tubulin Associated Proteins

• Tubulin polymerisation HTS assay using >97% pure tubulin,
  OD based | UK Prices
• Tubulin polymerisation HTS assay using >99% pure tubulin,
  fluorescence based | UK Prices
• Tubulin polymerisation HTS assay using >99% pure tubulin
  OD based | UK Prices
• Tubulin ligand competition assay | UK Prices

Tubulin Buffers, Reagents & MAPs

• Cytoskelfix™ Cell Fixative | UK Prices
• General tubulin buffer | UK Prices
• GTP (100mM stock) | UK Prices
• Tubulin glycerol buffer | UK Prices
• Microtubule associated protein (MAP) fraction | UK Prices
• Paclitaxel (2mM stock) | UK Prices
• Tau protein, bovine brain, lyophilised | UK Prices

Kinesin Motor Proteins

Purified Kinesin Motor Proteins

• Preformed microtubules for kinesin ATPase assays | UK Prices
• CENP-E kinesin motor domain protein (H.sapiens) | UK Prices
• Chromokinesin kinesin motor domain protein (H.sapiens) | UK Prices
• Eg5 kinesin motor domain protein (H.sapiens) | UK Prices
• Eg5 homolog BimC motor domain protein (A.nidulans) | UK Prices
• Eg5 homolog BimC motor domain protein (A.fumigatus) | UK Prices
• Eg5 homolog Kip1 motor domain protein (C.albicans) | UK Prices
• KIFC3 motor domain protein (H.sapiens) | UK Prices
• KIF3C motor domain protein (H.sapiens) | UK Prices
• Kinesin heavy chain motor domain protein (H.sapiens) | UK Prices
• MCAK motor domain protein (H.sapiens) | UK Prices
• MKLP motor domain protein (H.sapiens) | UK Prices
• Myosin II skeletal muscle protein (rabbit) | UK Prices
• Heavy meromyosin skeletal muscle protein (rabbit) | UK Prices

Kinesin Motor Protein Antibodies

• Kinesin heavy chain antibody
  (rabbit polyclonal, Drosophila antigen) | UK Prices
• Kinesin heavy chain antibody
  (mouse monoclonal, clone SUK4, sea urchin antigen) | UK Prices
• Eg5 antibody
  (rabbit polyclonal, human motor domain protein) | UK Prices
• CENP-E antibody
  (rabbit polyclonal, human motor domain protein) | UK Prices
• MCAK antibody
  (rabbit polyclonal, human motor domain protein) | UK Prices
• MKLP1 antibody
  (rabbit polyclonal, human motor domain protein) | UK Prices
• KIF3C antibody
  (rabbit polyclonal, human stalk region antigen) | UK Prices
• KIF1C antibody
  (rabbit polyclonal, human stalk region antigen) | UK Prices
• Kid antibody
  (rabbit polyclonal, human stalk region antigen) | UK Prices
• Hklp2 antibody
  (rabbit polyclonal, human stalk region antigen) | UK Prices

Kinesin Motor Protein Biochem Kits™

• Kinesin / Dynein motility Kit, fluorescence | UK Prices
• Kinesin ELIPA™ Kit | UK Prices
• Kinase HTS ATPase end point assay | UK Prices
• Microtubule stabilisation / destabilisation assay | UK Prices

Other Motor Protein Reagents

• Microtubles, preformed, lyophilised | UK Prices
• Paclitaxel (2mM stock) | UK Prices
• Perfusion chambers | UK Prices
• Rhodamine phalloidin | UK Prices

Intermediate Filament Proteins

• Vimentin Recombinant | UK Prices

Intermediate Filament Biochem Kits™

• Vimentin Biochem Kit | UK Prices
• Cytoskeleton Isolation Kit | UK Prices

Small G-proteins and Signal Transduction Products

Cytoskeleton, Inc. provides a wide range of purified G-proteins, regulators and effectors as well as the most comprehensive array of G-protein signal transduction assay kits.

GTPases

• Cdc42 protein, GST-tagged, wild type | UK Prices
• Cdc42 protein, GST-tagged, wild type on agarose beads | UK Prices
• Cdc42 protein, His-tagged, wild type | UK Prices
• Cdc42 protein, GST-tagged, constitutively active | UK Prices
• Cdc42 protein, GST-tagged, constitut. active on agarose beads | UK Prices
• Cdc42 protein, His-tagged, constitutively active | UK Prices
• Cdc42 protein, GST-tagged, dominant negative | UK Prices
• H-ras protein, His-tagged, wild type (His-H-ras) | UK Prices
• H-ras protein, GST-tagged, constitutively active | UK Prices
• H-ras protein, GST-tagged, constit. active on agarose beads | UK Prices
• Rac1 protein , GST-tagged, wild type (GST-Rac1) | UK Prices
• Rac1 protein, GST-tagged, wild type, on agarose beads (GST-Rac1 beads) | UK Prices
• Rac1 protein, His-tagged, wild type (His-Rac1) | UK Prices
• Rac1 protein, GST-tagged, constitutively active | UK Prices
• Rac1 protein, GST-tagged, constitut. active, on agarose beads | UK Prices
• Rac1 protein, His-tagged, constitutively active | UK Prices
• Rac1 protein, GST-tagged, dominant negative | UK Prices
• Ran protein, His-tagged, wild type (His-Ran) | UK Prices
• Ran protein, His-tagged, constitutively active | UK Prices
• Ran protein, His-tagged, dominant negative | UK Prices
• Rap 1a protein, His-tagged, wild type | UK Prices
• Rap 1b protein, His-tagged, wild type | UK Prices
• RhoA protein, GST-tagged, wild type (GST-RhoA) | UK Prices
• RhoA protein, GST-tagged, wild type on agarose beads (GST-RhoA beads) | UK Prices
• RhoA protein, His-tagged, wild type (His-RhoA) | UK Prices
• RhoA protein, GST-tagged, constitut. active, on agarose beads | UK Prices
• RhoA protein, His-tagged, constitutively active | UK Prices
• RhoC protein, His-tagged, wild type (His-RhoC) | UK Prices

Effector Fragments

• PAK p21-binding domain protein, GST-tagged | UK Prices
• PAK p21-binding domain protein on agarose beads, GST-tagged | UK Prices
• Raf Ras-binding domain protein on agarose beads, GST-tagged | UK Prices
• RanBP1 protein, His-tagged | UK Prices
• Rhotekin Rho-binding domain protein, GST-tagged | UK Prices
• Rhotekin Rho-binding domain protein on agarose beads, GST-tagged | UK Prices
• WASP Cdc42-binding domain protein on agarose beads, GST-tagged | UK Prices

GTPase Activating Protein (GAPs)

• p50RhoGAP protein, GST-tagged, full length | UK Prices
• p50RhoGAP protein, GST-tagged, catalytic domain | UK Prices

Quanine Nucleotide Dissociation Inhibitors (GDIs)

• Rho guanine nucleotide dissociation inhibitor protein-1, GST-tagged | UK Prices

Quanine Nucleotide Exchange Factors (GEFs)

• Dbs RhoGEF domain protein, His-tagged | UK Prices

Modifying Enzymes

• C3 transferase protein | UK Prices
• Cell permeable C3 transferase | UK Prices

Antibodies to G-proteins and related molecules

• Cdc42, sheep polyclonal, peptide human | UK Prices
• Rac1, rabbit polyclonal, peptide | UK Prices
• Rac1 specific, mouse monoclonal | UK Prices
• Ras, mouse monoclonal | UK Prices
• RhoA, mouse monoclonal | UK Prices
• WASP, rabbit polyclonal | UK Prices
• Sheep IgG HRP, donkey polyclonal, secondary antibody | UK Prices
• Sheep IgG Rhodamine, donkey polyclonal, secondary antibody | UK Prices

Small G-protein and Signal Transduction Biochem Kits™

• Cdc42 activation assay | UK Prices
• EasyRad™ phosphate assay | UK Prices
• G-LISA™ RhoA activation assay, absorbance based | UK Prices
• G-LISA™ RhoA activation assay, luminometry based | UK Prices
• G-LISA™ Cdc42 activation assay (colorimetric format) | UK Prices
• G-LISA™ Rac1 activation assay, luminometry based | UK Prices
• G-LISA™ Rac 1,2,3 activation assay, colorimetric format | UK Prices
• Rac activation assay | UK Prices
• Ras activation assay | UK Prices
• RhoA activation assay | UK Prices
• RhoGAP assay | UK Prices
• RhoGEF exchange assay | UK Prices

CellVision™ Prepared Cells on Coverslips

Cells are presented as a fixed and lyophilised preparation on a coverslip ready for your antibody or chemical probe. These preparations save on the time of cell culturing and the cost of growth factors and inconsistent growth conditions.

• Cells for the Rho Signalling Pathway, Apoptosis and Cytokinesis. | UK Prices

Biochem Kits™

Actin Biochem Kits™ (see under Actin Proteins)
Tubulin Biochem Kits™ (see under Tubulin Proteins)
Kinesin Motor Protein Biochem Kits™ (see under Kinesin Motor Proteins)

Phosphate Quantitation Biochem Kits™

• ATPase ELIPA™ Kit | UK Prices
• CytoPhos™ kinetic phosphate assay | UK Prices
• EasyRad™ phosphate assay | UK Prices
• GTPase ELIPA™ Kit | UK Prices
• PhosFree™ phosphate assay | UK Prices

Small G-protein and Signal Transduction Kits™ (see under Small G-protein/Signal Transduction Products)

Advanced Protein Assays™

• Advanced Protein Assay (high sensitivity) | UK Prices
• Precision Red Advanced Protein Assay (broad range) | UK Prices

Drug Discovery / High Throughput CytoDYNAMIX Screens™

CytoDYNAMIX Screens™ are highly robust high throughput assays for use in drug discovery.
Cytoskeleton offers a variety of high throughput assays that can be used on a small scale for verification and general research purposes.

HTS Assays for ATPases, GTPases or phosphate measurements

• Measurement of Pi generation by ATPases and GTPases is a simple, cost effective functional assay for this class of important enzymes. Cytoskeleton Inc provide several NTPase assays for HTS applications.

HTS Assays for Actin and Actin Binding Proteins

• The actin CytoDYNAMIX Screen™ series are high throughput drug development screens based on actin proteins, isotypes of actin and actin binding proteins such as WASP, cofilin and profiling.

HTS Assays for Motor Proteins

CytoDYNAMIX Screens

• The Cytoskeleton Motor Werks™ range of products provides a comprehensive range of motor proteins, antibodies and assays that aid motor protein research.

HTS Assays for Small G-proteins

CytoDYNAMIX Screens

• HTS format assays to measure in vivo activities of small G-proteins as well as in vitro drug discovery HTS assays for GEFs, GAPs and G-protein-effector interactions.

HTS Assays for Tubulin and Tubulin associated proteins

CytoDYNAMIX Screens

• Cytoskeleton Inc are world leaders at providing tubulin proteins and tubulin related assays.
• Polymerisation assays in fluorescence and optical density formats offer the most flexible assay system for identifying and measuring tubulin ligands. New specialised tubulins have also been introduced for anti-cancer and anti-fungal drug development.